plac plasmid backbone Search Results


library transfer vector plas  (Addgene inc)
95
Addgene inc library transfer vector plas
Library Transfer Vector Plas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library transfer vector plas/product/Addgene inc
Average 95 stars, based on 1 article reviews
library transfer vector plas - by Bioz Stars, 2026-03
95/100 stars
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sgrna sequences  (Addgene inc)
95
Addgene inc sgrna sequences
A Schematic of screen workflow. B , C , D Volcano plots of IgG1 + iGC ( B ), IgE + iGC ( C ), IgE + PC ( D <t>)</t> <t>CRISPR</t> screens, showing CRISPR score (average <t>sgRNA</t> enrichment or depletion per gene) vs statistical significance corrected for false discover rate (FDR) for all genes (grey) and expected essential genes (dark red). E , F Schematics showing CD40 and IL-4 signaling pathways ( E ), BCR and TNFRSF13C signaling pathways ( F ), indicating minimum FDR (≤ 0.01 dark red, ≤ 0.05 orange, ≤ 0.25 yellow) across all screens for indicated gene products. For minimum FDR > 0.25, or where genes are not targeted in the CRISPR screen (PLCG2), signaling pathway components are indicated in grey.
Sgrna Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna sequences/product/Addgene inc
Average 95 stars, based on 1 article reviews
sgrna sequences - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
dcas9 vp64  (Addgene inc)
93
Addgene inc dcas9 vp64
A Schematic of screen workflow. B , C , D Volcano plots of IgG1 + iGC ( B ), IgE + iGC ( C ), IgE + PC ( D <t>)</t> <t>CRISPR</t> screens, showing CRISPR score (average <t>sgRNA</t> enrichment or depletion per gene) vs statistical significance corrected for false discover rate (FDR) for all genes (grey) and expected essential genes (dark red). E , F Schematics showing CD40 and IL-4 signaling pathways ( E ), BCR and TNFRSF13C signaling pathways ( F ), indicating minimum FDR (≤ 0.01 dark red, ≤ 0.05 orange, ≤ 0.25 yellow) across all screens for indicated gene products. For minimum FDR > 0.25, or where genes are not targeted in the CRISPR screen (PLCG2), signaling pathway components are indicated in grey.
Dcas9 Vp64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dcas9 vp64/product/Addgene inc
Average 93 stars, based on 1 article reviews
dcas9 vp64 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
pevol plasmid backbones  (Addgene inc)
92
Addgene inc pevol plasmid backbones
A Schematic of screen workflow. B , C , D Volcano plots of IgG1 + iGC ( B ), IgE + iGC ( C ), IgE + PC ( D <t>)</t> <t>CRISPR</t> screens, showing CRISPR score (average <t>sgRNA</t> enrichment or depletion per gene) vs statistical significance corrected for false discover rate (FDR) for all genes (grey) and expected essential genes (dark red). E , F Schematics showing CD40 and IL-4 signaling pathways ( E ), BCR and TNFRSF13C signaling pathways ( F ), indicating minimum FDR (≤ 0.01 dark red, ≤ 0.05 orange, ≤ 0.25 yellow) across all screens for indicated gene products. For minimum FDR > 0.25, or where genes are not targeted in the CRISPR screen (PLCG2), signaling pathway components are indicated in grey.
Pevol Plasmid Backbones, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pevol plasmid backbones/product/Addgene inc
Average 92 stars, based on 1 article reviews
pevol plasmid backbones - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
plac plasmid backbone  (Addgene inc)
92
Addgene inc plac plasmid backbone
A Schematic of screen workflow. B , C , D Volcano plots of IgG1 + iGC ( B ), IgE + iGC ( C ), IgE + PC ( D <t>)</t> <t>CRISPR</t> screens, showing CRISPR score (average <t>sgRNA</t> enrichment or depletion per gene) vs statistical significance corrected for false discover rate (FDR) for all genes (grey) and expected essential genes (dark red). E , F Schematics showing CD40 and IL-4 signaling pathways ( E ), BCR and TNFRSF13C signaling pathways ( F ), indicating minimum FDR (≤ 0.01 dark red, ≤ 0.05 orange, ≤ 0.25 yellow) across all screens for indicated gene products. For minimum FDR > 0.25, or where genes are not targeted in the CRISPR screen (PLCG2), signaling pathway components are indicated in grey.
Plac Plasmid Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plac plasmid backbone/product/Addgene inc
Average 92 stars, based on 1 article reviews
plac plasmid backbone - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
pcclc mnd a0201 sabr backbone plasmid 119050  (Addgene inc)
N/A
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lenti sgrna ms2 puro optimized backbone plasmid 73797  (Addgene inc)
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pc016 lwcas13a guide expression backbone with u6 promoter plasmid 91906  (Addgene inc)
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crispseq bfp backbone plasmid 85707  (Addgene inc)
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rna motif plasmid cloning backbone plasmid 107253  (Addgene inc)
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pxr003 casrx grna cloning backbone plasmid 109053  (Addgene inc)
N/A
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px210 u6 pre crrna backbone ef1a hspcas9 plasmid 41861  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


A Schematic of screen workflow. B , C , D Volcano plots of IgG1 + iGC ( B ), IgE + iGC ( C ), IgE + PC ( D ) CRISPR screens, showing CRISPR score (average sgRNA enrichment or depletion per gene) vs statistical significance corrected for false discover rate (FDR) for all genes (grey) and expected essential genes (dark red). E , F Schematics showing CD40 and IL-4 signaling pathways ( E ), BCR and TNFRSF13C signaling pathways ( F ), indicating minimum FDR (≤ 0.01 dark red, ≤ 0.05 orange, ≤ 0.25 yellow) across all screens for indicated gene products. For minimum FDR > 0.25, or where genes are not targeted in the CRISPR screen (PLCG2), signaling pathway components are indicated in grey.

Journal: bioRxiv

Article Title: Genome-wide screens identify calcium signaling as a key regulator of IgE + plasma cell differentiation and survival

doi: 10.1101/2021.03.02.433398

Figure Lengend Snippet: A Schematic of screen workflow. B , C , D Volcano plots of IgG1 + iGC ( B ), IgE + iGC ( C ), IgE + PC ( D ) CRISPR screens, showing CRISPR score (average sgRNA enrichment or depletion per gene) vs statistical significance corrected for false discover rate (FDR) for all genes (grey) and expected essential genes (dark red). E , F Schematics showing CD40 and IL-4 signaling pathways ( E ), BCR and TNFRSF13C signaling pathways ( F ), indicating minimum FDR (≤ 0.01 dark red, ≤ 0.05 orange, ≤ 0.25 yellow) across all screens for indicated gene products. For minimum FDR > 0.25, or where genes are not targeted in the CRISPR screen (PLCG2), signaling pathway components are indicated in grey.

Article Snippet: The Cherry Brie pooled CRISPR library was obtained by Gibson assembly cloning to place the sgRNA sequences form the Mouse Brie CRISPR knockout pooled library (a gift from David Root and John Doench (Addgene #73633)) into the LentiGuide-Cherry plasmid (Supplementary Figure 1A).

Techniques: CRISPR, Protein-Protein interactions